Assay characteristics:
| Cat.No.: | BI-20462 |
| Method: | Sandwich ELISA, HRP/TMB, 12x8-well strips |
| Sample type: | Serum, heparin plasma |
| Standard range: | 0-2 pmol/l (7 serum based standards) |
| Standard points: | 0/0.0625/0.125/0.25/0.5/1/2 pmol/l |
| Controls: | 2 serum based controls |
| Sample size: | 150 μl / well |
| Incubation time: | 2 h / overnight / 1 h / 30 min |
| Unit conversion: | 1 pg/ml = 0.05 pmol/l (MW: 20 kDa, monomer) |
Sensitivity:
LOD: 0.01 pmol/l (0 pmol/l + 3 SD); LLOQ: 0.008 pmol/l
Precision:
Intra-assay (n=5) ≤ 5%, Inter-assay (n=12) ≤ 3%
Spike/Recovery:
The mean recovery of endogenous free sRANKL (spike: 0.15 pmol/l; 0.34 pmol/l) is:
Serum: 89%; 85%
Heparin plasma: 100%; 98%
Dilution linearity:
Dilution linearity of endogenous free sRANKL with a low measuring serum as dilution medium is:
Serum: 1+1 dilution = 112%, 1+3 dilution = 121%
Heparin plasma: 1+1 dilution = 121%
Cross reactivity:
The sequence homology to various primates is >95%. It is likely that the assay can be used for these species. Internal validations have not been carried out.
Values from apparently healthy individuals:
Median (serum, n=32): 0.14 pmol/l.
It is recommended to establish the normal range for each laboratory.
Principle of the assay:
Manual ELISAs easily adaaptable for automation!